典型文献
Molecular Epidemiology of Klebsiella pneumoniae from Clinical Bovine Mastitis in Northern Area of China,2018-2019
文献摘要:
Klebsiella pneumoniae(K.pneumonia,KpⅠ)is a predominate inducement of bovine mastitis,which is asso-ciated with high mortality and milk yield reduction.However,data is lacking on the molecular character-istics of bovine K.pneumoniae,limiting the risk assessment of its transmission through the food chain.Herein,we investigated the prevalence of K.pneumoniae in 6301 clinical mastitis(CM)milk samples from dairy cattle in northern area of China.In total,183 K.pneumoniae isolates were recovered,with detection rates of 3.0%and 2.8%in 2018 and 2019,respectively.Like human clinical K.pneumoniae,all CM K.pneu-moniae isolates belonged to one of three phylogroups:KpⅠ(n=143),Klebsiella.quasipneumoniae subsp.similipneumoniae(KpⅡ-B)(n=37),and Klebsiella variicola(KpⅢ)(n=3).We detected the extended-spectrumβ-lactamase-encoding genes blaSHV-2a,blaCTX-M-14,and blaCTX-M-15,as well as clpC,lpfA,lacI,lacZ,lacY,and the fecABDEIR operon in the KpⅠ isolates,which may contribute to their pathogenicity and host adaptability in cows.The high prevalence of KpⅠ in dairy farms may be problematic,as it showed relatively higher rates of antibiotic resistance and virulence gene carriage than the KpⅡ-B and KpⅢ iso-lates.Furthermore,we observed distinct differences in population structure between CM-and human infection-associated KpⅠ isolates,with the genes associated with invasive infection in humans rarely being observed in bovine isolates,indicating that few CM-associated K.pneumoniae isolates pose a threat to human health.Nevertheless,bovine KpⅡ-B isolates shared a high level of nucleotide sequence identity with isolates from human infections and frequently carried the nitrogen-fixation gene nif,suggesting an association between KpⅡ-B isolates from cattle and humans,and plant-derived bacteria.
文献关键词:
中图分类号:
作者姓名:
Shikai Song;Wenjuan He;Dawei Yang;Manar Benmouffok;Yao Wang;Jiyun Li;Chengtao Sun;Xiangbin Song;Shizhen Ma;Chang Cai;Shuangyang Ding;Congming Wu;Zhangqi Shen;Yang Wang
作者机构:
Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety,College of Veterinary Medicine,China Agricultural University,Beijing 100193,China;China Institute of Veterinary Drug Control,Beijing 100081,China;College of Veterinary Medicine,Hunan Agricultural University,Changsha 410125,China;Research and Innovation Office, Murdoch University, Murdoch 6150, Australia;China Australia Joint Laboratory for Animal Health Big Data Analytics,College of Animal Science and Technology,Zhejiang Agricultural and Forestry University,Hangzhou 311300,China
文献出处:
引用格式:
[1]Shikai Song;Wenjuan He;Dawei Yang;Manar Benmouffok;Yao Wang;Jiyun Li;Chengtao Sun;Xiangbin Song;Shizhen Ma;Chang Cai;Shuangyang Ding;Congming Wu;Zhangqi Shen;Yang Wang-.Molecular Epidemiology of Klebsiella pneumoniae from Clinical Bovine Mastitis in Northern Area of China,2018-2019)[J].工程(英文),2022(03):146-154
A类:
phylogroups,quasipneumoniae,similipneumoniae,variicola,clpC,lacI,lacY,fecABDEIR,nif
B类:
Molecular,Epidemiology,Klebsiella,from,Clinical,Bovine,Mastitis,Northern,Area,China,Kp,predominate,inducement,bovine,mastitis,which,mortality,milk,yield,reduction,However,data,lacking,molecular,character,istics,limiting,risk,assessment,its,transmission,through,food,chain,Herein,investigated,prevalence,clinical,CM,samples,dairy,cattle,northern,area,In,total,isolates,were,recovered,detection,rates,respectively,Like,all,belonged,one,three,subsp,We,detected,extended,spectrum,lactamase,encoding,genes,blaSHV,2a,blaCTX,well,lpfA,lacZ,operon,may,contribute,their,pathogenicity,host,adaptability,cows,farms,problematic,showed,relatively,higher,antibiotic,resistance,virulence,carriage,than,Furthermore,observed,distinct,differences,population,structure,between,associated,invasive,humans,rarely,being,indicating,that,few,pose,threat,health,Nevertheless,shared,level,nucleotide,sequence,identity,infections,frequently,carried,nitrogen,fixation,suggesting,association,plant,derived,bacteria
AB值:
0.499382
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