Objective: To evaluate the levels of estrogen, albumin and gonadotropins (luteinizing hormone, follicle-stimulating hormone) as well as the activity of dopamine beta hydroxylase (DAβ H) in aged female rats treated with nitric oxide precursor L-arginine and neuronal nitric oxide synthase antagonist L-NAME.Methods: A total of 224 Wistar rats (36 weeks old, weighing 250 g) based on a random sampling were divided into the control and experimental groups after Pap smear test. The control group received only saline (1 mL/kg) intraperitoneally (i.p.). The experiential groups were treated with L-arginine (5, 25 and 50 mg/kg, i.p.) and L-NAME (5 and 25 mg/kg, i.p.) for 3 to 21 days, once a day. Blood samples were taken from the rats and the levels of estrogen and albumin and gonadotropins in the serum were monitored by enzyme-linked immunosorbent assay kit, and the ovaries were examined immunohistopathologically for DAβH activity. Results: L-arginine (5 mg/kg) significantly increased estrogen level (P<0.05), which was associated with DAβH activation in the ovaries. L-NAME reduced this effect when administered prior to L-arginine dose. L-arginine caused no significant change in the levels of luteinizing hormone and follicle-stimulating hormone. Except for the lowest dose of L-arginine in the shortest period, albumin levels significantly decreased in other treatments compared to the control group (P<0.05). Conclusions: L-arginine is likely to reduce postmenopausal problems due to an increased nitric oxide level.

Fatemeh Lakzaei;Manizheh Karami;Mohammadreza Jalali Nadoushan

Department of Biology,Faculty of Basic Sciences,Shahed University,Tehran,Iran;Department of Pathology,Faculty of Medicine,Shahed University,Tehran,Iran

亚太生殖杂志（英文版）

[1]Fatemeh Lakzaei;Manizheh Karami;Mohammadreza Jalali Nadoushan-.L-arginine alleviates postmenopausal complications in female rats by stimulating ovarian dopamine beta hydroxylase)[J].亚太生殖杂志（英文版）,2022(06):276-284

Pap,immunohistopathologically

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