The retina is a complex visual tissue that perceives light signals because of an exquisite orchestration of high cellular complexity(Fig.1A).It is of great significance to explore the transcriptome diver-sity of each cell type for comprehensive understanding(Voigt et al.,2020b).Unlike conventional sequencing methods,single-cell RNA-sequencing(scRNA-seq)and single-nucleus RNA-sequencing(snRNA-seq)accurately profile transcriptomic characterization of in-dividual whole cells and nuclei,respectively.Especially,closely related neuronal cell types can be similarly discriminated by these two sequencing methods(Bakken et al.,2018;Hedlund and Deng,2018).The application of scRNA-seq has produced retina datasets of several species,including human(Voigt et al.,2020a),macaque(Peng et al.,2019),mouse(Hoang et al.,2020),chick(Yamagata et al.,2021),Xenopus,and zebrafish(Hoang et al.,2020).However,establishing a comprehensive understanding of retinas across multi-ple species is challenging due to cellular heterogeneity(Peng et al.,2019).The lack of a multispecies retinal single-cell dataset platform impedes the identification of orthologous cell types across species.Here,we apply snRNA-seq technology to profile the retina cellular heterogeneity of four species that were not previously reported,as well as establish a dedicated platform for retina single-cell atlases,including ten species.

College of Life Sciences,University of Chinese Academy of Sciences,Beijing 100049,China;BGI-Shenzhen,Shenzhen,Guangdong 518083,China;Department of Computer Science,City University of Hong Kong,Hong Kong 999077,China;Tsinghua-Berkeley Shenzhen Institute(TBSI),Tsinghua University,Shenzhen,Guangdong 518055,China;Department of Physiology,School of Basic Medical Sciences,Binzhou Medical University,Yantai,Shandong 264003,China;Fujian Key Laboratory of Developmental and Neural Biology,College of Life Sciences,Fujian Normal University,Fuzhou,Fujian 350117,China;Cuiying Biomedical Research Center,Lanzhou University Second Hospital,Lanzhou,Gansu 730010,China;Institute of Pediatrics,Department of Pediatric Surgery,Shenzhen Children's Hospital,Shenzhen,Guangdong 518038,China;Department of Pediatrics,The Affiliated Hospital of Guangdong Medical University,Zhanjiang,Guangdong 524001,China

[1]Zaoxu Xu;Changzheng Wang;Min Chen;Yuting Yuan;Linzhou Li;Zhen Huang;Yue Yuan;Huanming Yang;Qi Wang;Xingliang Zhang-.Retina cell atlases of multiple species and an online platform for retina cell-type markers)[J].遗传学报,2022(03):262-265

atlases,Hedlund,Hoang

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