典型文献
BMP4 preserves the developmental potential of mESCs through Ube2s-and Chmp4b-mediated chromosomal stability safeguarding
文献摘要：
Chemically defined medium is widely used for culturing mouse embryonic stem cells(mESCs),in which N2B27 works as a substitution for serum,and GSK3β and MEK inhibitors(2i)help to promote ground-state pluripo-tency.However,recent studies suggested that MEKi might cause irreversible defects that compromise the developmental potential of mESCs.Here,we demon-strated the deficient bone morphogenetic protein(BMP)signal in the chemically defined condition is one of the main causes for the impaired pluripotency.Mechanisti-cally,activating the BMP signal pathway by BMP4 could safeguard the chromosomal integrity and proliferation capacity of mESCs through regulating downstream tar-gets Ube2s and Chmp4b.More importantly,BMP4 pro-motes a distinct in vivo developmental potential and a long-term pluripotency preservation.Besides,the pluripotent improvements driven by BMP4 are superior to those by attenuating MEK suppression.Taken together,our study shows appropriate activation of BMP signal is essential for regulating functional pluripotency and reveals that BMP4 should be applied in the serum-free culture system.
文献关键词：
中图分类号：
作者姓名：
Mingzhu Wang;Kun Zhao;Meng Liu;Mengting Wang;Zhibin Qiao;Shanru Yi;Yonghua Jiang;Xiaochen Kou;Yanhong Zhao;Jiqing Yin;Tianming Li;Hong Wang;Cizhong Jiang;Shaorong Gao;Jiayu Chen
作者机构：
Clinical and Translation Research Center of Shanghai First Maternity&Infant Hospital,Shanghai Key Laboratory of Signaling and Disease Research,School of Life Sciences and Technology,Tongji University,Shanghai 200092,China;Frontier Science Center for Stem Cell Research,Tongji University,Shanghai 200092,China;Key Laboratory of Spine and Spinal Cord Injury Repair and Regeneration of Ministry of Education,Orthopaedic Department of Tongji Hospital,Tongji University,Shanghai 200065,China;Institute for Regenerative Medicine,Shanghai East Hospital,School of Life Sciences and Technology,Tongji University,Shanghai 200123,China;Guangxi Key Laboratory of Genomic and Personalized Medicine,Nanning 530021,China
文献出处：
引用格式：
[1]Mingzhu Wang;Kun Zhao;Meng Liu;Mengting Wang;Zhibin Qiao;Shanru Yi;Yonghua Jiang;Xiaochen Kou;Yanhong Zhao;Jiqing Yin;Tianming Li;Hong Wang;Cizhong Jiang;Shaorong Gao;Jiayu Chen-.BMP4 preserves the developmental potential of mESCs through Ube2s-and Chmp4b-mediated chromosomal stability safeguarding)[J].蛋白质与细胞,2022(08):580-601
A类：
mESCs,Ube2s,Chmp4b,N2B27,pluripo,MEKi,Mechanisti
B类：
BMP4,preserves,developmental,potential,through,mediated,chromosomal,stability,safeguarding,Chemically,defined,medium,widely,used,culturing,mouse,embryonic,cells,which,works,as,substitution,serum,GSK3,inhibitors,2i,help,promote,ground,state,However,recent,studies,suggested,that,might,irreversible,defects,compromise,Here,demon,strated,deficient,bone,morphogenetic,protein,signal,chemically,condition,main,causes,impaired,pluripotency,activating,pathway,by,could,integrity,proliferation,capacity,regulating,downstream,tar,gets,More,importantly,motes,distinct,vivo,long,term,preservation,Besides,pluripotent,improvements,driven,are,superior,those,attenuating,suppression,Taken,together,our,study,shows,appropriate,activation,essential,functional,reveals,should,applied,free,culture,system
AB值：
0.558407
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