典型文献
A Photocaged Azidosugar for Light-Controlled Metabolic Labeling of Cell-Surface Sialoglycans
文献摘要：
The photocaging strategy has been widely used for activating biomolecules and hence regulating the underlying biological processes with high temporal and spatial resolution.Herein,we integrate this strategy into metabolic glycan labeling,which enables optical-ly-controlled metabolic labeling of cell-surface sialoglycans with azides.N-Azidoacetylmannosamine(ManNAz)is caged with a 4,5-dimethoxy-2-nitrobenzyl(DMNB)group at the anomeric hydroxyl group to block its cellular metabolism.After feeding to the cells,irradiation with 365 nm UV light or 405 nm visible light cleaves the photocage and generates ManNAz within the cells.ManNAz is metabolically converted to azido sialic acid,which is then incorporated into cell-surface sialoglycans.We demonstrated spatial and temporal control of this strategy by specifically labeling sialoglycans on a single cell and tracking the metabolism of ManNAz based on light-triggered pulse-chase labeling,respectively.The light-controlled metabolic glycan labeling strategy should facilitate probing of glycosylation in complex biological systems with high spatiotemporal precision.
文献关键词：
中图分类号：
作者姓名：
Bo Cheng;Yi Wan;Qi Tang;Yifei Du;Feiyang Xu;Zhimin Huang;Wei Qin;Xing Chen
作者机构：
College of Chemistry and Molecular Engineering,Peking University,Beijing 100871,China;Beijing National Laboratory for Molecular Sciences,Peking University,Beijing 100871,China;Peking-Tsinghua Center for Life Sciences,Peking University,Beijing 100871,China;Synthetic and Functional Biomolecules Center Peking University,Beijing 100871,China;Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education,Peking University,Beijing 100871,China
文献出处：
引用格式：
[1]Bo Cheng;Yi Wan;Qi Tang;Yifei Du;Feiyang Xu;Zhimin Huang;Wei Qin;Xing Chen-.A Photocaged Azidosugar for Light-Controlled Metabolic Labeling of Cell-Surface Sialoglycans)[J].中国化学（英文版）,2022(07):806-812
A类：
Photocaged,Azidosugar,Sialoglycans,photocaging,sialoglycans,Azidoacetylmannosamine,ManNAz,nitrobenzyl,DMNB,anomeric,cleaves,photocage,azido
B类：
Light,Controlled,Metabolic,Labeling,Cell,Surface,strategy,been,widely,used,activating,biomolecules,hence,regulating,underlying,biological,processes,high,spatial,resolution,Herein,we,integrate,this,into,labeling,which,enables,optical,controlled,surface,azides,dimethoxy,group,hydroxyl,block,its,cellular,metabolism,After,feeding,cells,irradiation,UV,light,visible,generates,within,metabolically,converted,sialic,acid,then,incorporated,We,demonstrated,by,specifically,single,tracking,triggered,pulse,chase,respectively,should,facilitate,probing,glycosylation,complex,systems,spatiotemporal,precision
AB值：
0.447944
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