A novel salt-tolerant heterotrophic nitrification and aerobic denitrification(HN-AD)bacterium was isolated and identified as Halomonas venusta TJPU05(H.venusta TJPU05).The nitrogen removal performance of H.venusta TJPU05 in simulated water(SW)with sole or mixed nitrogen sources and in actual wastewater(AW)with high concentration of salt and nitrogen was investigated.The results showed that 86.12％of NH4+-N,95.68％of NO3--N,100％of NO2--N and 84.57％of total nitrogen(TN)could be removed from SW with sole nitrogen sources within 24 h at the utmost.H.venusta TJPU05 could maximally remove 84.06％of NH4+-N,92.33％of NO3--N,92.9％of NO2--N and 77.73％of TN from SW with mixed nitrogen source when the salinity was above 8％.The application of H.venusta TJPU05 in treating AW with high salt and high ammonia nitrogen led to removal efficiencies of 50.96％,47.28％and 43.19％for NH4+-N,NO3--N and TN respectively without any optimization.Furthermore,the activities of nitrogen removal-related enzymes of the strain were also investigated.The successful detection of high level activities of ammonia oxygenase(AMO),hydroxylamine oxidase(HAO),nitrate reductase(NAR)and nitrite reductase(NIR)enzymes under high salinity condition further proved the HN-AD and salt-tolerance capacity of H.venusta TJPU05.These results demonstrated that the H.venusta TJPU05 has great potential in treating high-salinity nitrogenous wastewater.

Quanli Man;Peilian Zhang;Weiqi Huang;Qing Zhu;Xiaoling He;Dongsheng Wei

State Key Laboratory of Separation Membrane and Membrane Process,School of Environmental Science and Engineering,Tiangong University,Tianjin 300387,China;School of Chemistry,Tiangong University,Tianjin 300387,China;Key Laboratory of Molecular Microbiology and Technology,Ministry of Education,College of Life Science,Nankai University,Tianjin 300071,China

环境科学与工程前沿

[1]Quanli Man;Peilian Zhang;Weiqi Huang;Qing Zhu;Xiaoling He;Dongsheng Wei-.A heterotrophic nitrification-aerobic denitrification bacterium Halomonas venusta TJPU05 suitable for nitrogen removal from high-salinity wastewater)[J].环境科学与工程前沿,2022(06):13-22

TJPU05

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