Lake mixing influences aquatic chemical properties and microbial community composition,and thus,we hypothesized that it would alter microbial community assembly and interac-tion.To clarify this issue,we explored the community assembly processes and cooccurrence networks in four seasons at two depths(epilimnion and hypolimnion)in a mesotrophic and stratified lake(Chenghai Lake),which formed stratification in the summer and turnover in the winter.During the stratification period,the epilimnion and hypolimnion went through contrary assembly processes but converged to similar assembly patterns in the mixing pe-riod.In a highly homogeneous selection environment,species with low niche breadth were filtered,resulting in decreased species richness.Water mixing in the winter homogenized the environment,resulting in a simpler microbial cooccurrence network.Interestingly,we observed a high abundance of the cyanobacterial genus Planktothrix in the winter,proba-bly due to nutrient redistribution and Planktothrix adaptivity to the winter environment in which mixing played important roles.Our study provides deeper fundamental insights into how environmental factors influence microbial community structure through community assembly processes.

Junwen Chen;Huacong Liu;Yaohui Bai;Jing Qi;Weixiao Qi;Huijuan Liu;Jianfeng Peng;Jiuhui Qu

Center for Water and Ecology,State Key Joint Laboratory of Environment Simulation and Pollution Control,School of Environment,Tsinghua University,Beijing 100084,China;Key Laboratory of Drinking Water Science and Technology,Research Center for Eco-Environmental Sciences,Chinese Academy of Sciences,Beijing 100085,China

环境科学学报（英文版）

[1]Junwen Chen;Huacong Liu;Yaohui Bai;Jing Qi;Weixiao Qi;Huijuan Liu;Jianfeng Peng;Jiuhui Qu-.Mixing regime shapes the community assembly process,microbial interaction and proliferation of cyanobacterial species Planktothrix in a stratified lake)[J].环境科学学报（英文版）,2022(05):103-113

cooccurrence,epilimnion,hypolimnion,mesotrophic,Chenghai,riod

Mixing,regime,shapes,community,assembly,microbial,interaction,proliferation,cyanobacterial,species,Planktothrix,stratified,lake,Lake,mixing,influences,aquatic,chemical,properties,composition,thus,hypothesized,that,would,alter,To,clarify,this,issue,explored,processes,networks,four,seasons,depths,which,formed,stratification,summer,turnover,winter,During,period,went,through,contrary,converged,similar,patterns,highly,homogeneous,selection,low,niche,breadth,were,filtered,resulting,decreased,richness,Water,homogenized,simpler,Interestingly,observed,abundance,genus,proba,due,nutrient,redistribution,adaptivity,played,important,roles,Our,study,provides,deeper,fundamental,insights,into,how,environmental,factors,structure

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