Background: The majority of attenuated total reflection Fourier transform infrared (ATR FT-IR) investigations of cot-ton are focused on the fiber tissue for biological mechanisms and understanding of fiber development and maturity, but rarely on other cotton biomass components. This work examined in detail the ATR FT-IR spectral features of vari-ous cotton tissues/organs at reproductive and maturation stages, analyzed and discussed their biological implications. Results: The ATR FT-IR spectra of these tissues/organs were analyzed and compared with the focus on the lower wavenumber fingerprinting range. Six outstanding FT-IR bands at 1730, 1620, 1525, 1235, 1050 and 895 - cm?1 represented the major C=O stretching, protein Amide I, Amide Ⅱ, the O–H/N–H deformation, the total C–O–C stretch-ing and the β-glycosidic linkage in celluloses, respectively, and impacted differently between these organs with the two growth stages. Furthermore, the band intensity at 1620, 1525, 1235, and 1050 - cm?1 were exclusively and significantly correlated to the levels of protein (Amide I bond), protein (Amide Ⅱ bond), cellulose, and hemicellulose, respectively, whereas the band at 1730 - cm?1 was negatively correlated with ash content.Conclusions: The resulting observations indicated the capability of ATR FT-IR spectroscopy for monitoring changes, transportation, and accumulation of the major chemical components in these tissues over the cotton growth period. In other words, this spectral technology could be an effective tool for physiological, biochemical, and morphological research related to cotton biology and development.

HE Zhongqi;LIU Yongliang;KIM Hee Jin;TEWOLDE Haile;ZHANG Hailin

Southern Regional Research Center,USDA-ARS,New Orleans,LA 70124,USA;Crop Science Research Laboratory,USDA-ARS,Mississippi State,MS 39762,USA;Department of Plant and Soil Sciences,Oklahoma State University,Stillwater,OK 74078,USA

棉花研究（英文）

[1]HE Zhongqi;LIU Yongliang;KIM Hee Jin;TEWOLDE Haile;ZHANG Hailin-.Fourier transform infrared spectral features of plant biomass components during cotton organ development and their biological implications)[J].棉花研究（英文）,2022(02):16-28

celluloses

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