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典型文献
Absolute quantitative detection of genetically modified soybean MON87708×MON89788 with stacked traits by digital polymerase chain reaction
文献摘要:
The main advantage of digital PCR(dPCR)is that it facilitates absolute quantification of the target without reference to the standard/calibration curve.Crystal droplet dPCR has a three-color staining detection function,which enables multiplex PCR reaction.In this study,this technique was used to establish triple dPCR detection for the genetically modified soybean MON87708 x MON89788 with stacked traits.Specific absolute quantitative detection was accomplished for the genomic DNA extracted from the homogenized seeds of GM stack MON87708 x MON89788 soybean.Our results can serve as a reference for the absolute quantitative detection of stacked events of genetically modified crops.
文献关键词:
作者姓名:
Junyi Xu;Xin Li;Jinglian Bai;Ying Liu;Shaojie Wang;Yueting Liu;Chunguang Yang
作者机构:
Technology Center of Dalian Customs District,Changjiang East Road 60th,Dalian,China;Logistics Management Center of Dalian Customs District,Changjiang East Road 60th,Dalian,China
引用格式:
[1]Junyi Xu;Xin Li;Jinglian Bai;Ying Liu;Shaojie Wang;Yueting Liu;Chunguang Yang-.Absolute quantitative detection of genetically modified soybean MON87708×MON89788 with stacked traits by digital polymerase chain reaction)[J].中国油料作物学报(英文),2022(04):180-188
A类:
MON87708,MON89788
B类:
Absolute,quantitative,detection,genetically,modified,soybean,stacked,traits,by,digital,polymerase,chain,reaction,main,advantage,dPCR,that,facilitates,absolute,quantification,target,without,reference,standard,calibration,curve,Crystal,droplet,has,three,color,staining,function,which,enables,multiplex,In,this,study,technique,was,used,establish,triple,Specific,accomplished,genomic,extracted,from,homogenized,seeds,GM,Our,results,can,serve,events,crops
AB值:
0.524926
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