典型文献
Screening and identification of purine degrading Lactobacillus fermentum 9-4 from Chinese fermented rice-flour noodles
文献摘要：
The aim of this study was to isolate a new purine-degrading potential probiotic strain from Chinese fermented rice-flour noodles and investigate its potential application in purine-degrading food development for promising anti-gout therapy. A new lactic acid bacteria strain designated as Lactobacillus fermentum 9-4 was screened out from 10 Shengzhamifen samples by a comprehensive method integrating agar plate selection, in vitro purine-metabolizing enzymatic activities of xanthine oxidase and urate oxidase, 16S rRNA gene sequencing and identification. The resting cells of L. fermentum 9-4 showed the maximum degradation rates of inosine and guanosine by respective 2.13 × 10-3 and 2.78 × 10-3 g/(L·min), and the highest assimilation ratio of guanosine by (55.93 ± 3.12)％, which are improvements over LAB strains characterized previously. Yogurt fermented by L. fermentum 9-4 also efficiently assimilated the inosine and guanosine, with respective degradation rates of 98.10％ and 98.56％ higher than those of the commercial ones. The L. fermentum 9-4 showed excellent survival (> 80％) under the conditions of pH 2.5 and 0.1％ bile salt. The results suggest that L. fermentum 9-4 may be a promising candidate as a probiotic for developing low-purine foods.
文献关键词：
中图分类号：
作者姓名：
Lianghua Lu;Tiantian Liu;Xiaoling Liu;Chenghua Wang
作者机构：
College of Light Industry and Food Engineering,Guangxi University,Nanning 530004,China
文献出处：
引用格式：
[1]Lianghua Lu;Tiantian Liu;Xiaoling Liu;Chenghua Wang-.Screening and identification of purine degrading Lactobacillus fermentum 9-4 from Chinese fermented rice-flour noodles)[J].食品科学与人类健康（英文）,2022(05):1402-1408
A类：
noodles,Shengzhamifen,Yogurt
B类：
Screening,identification,purine,degrading,Lactobacillus,fermentum,from,Chinese,fermented,rice,flour,aim,this,study,was,isolate,new,potential,probiotic,investigate,its,application,development,promising,anti,gout,therapy,lactic,acid,bacteria,designated,screened,samples,by,comprehensive,method,integrating,agar,plate,selection,vitro,metabolizing,enzymatic,activities,xanthine,oxidase,urate,16S,rRNA,gene,sequencing,resting,cells,showed,maximum,degradation,rates,inosine,guanosine,respective,highest,assimilation,ratio,which,are,improvements,over,LAB,strains,characterized,previously,also,efficiently,assimilated,higher,than,those,commercial,ones,excellent,survival,under,conditions,bile,salt,results,suggest,that,may,be,candidate,developing,low,foods
AB值：
0.573735
相似文献
机标中图分类号，由域田数据科技根据网络公开资料自动分析生成，仅供学习研究参考。
