典型文献
Effects of silkworm pupa protein on apoptosis and energy metabolism in human colon cancer DLD-1 cells
文献摘要：
Objective: To investigate the effects of silkworm pupa (Bombyx mori) protein (SPP) on cell proliferation, apoptosis and energy metabolism in human colon cancer cells DLD-1. Methods: CCK-8 was used to detect cell proliferation rate after 72 h of cell culture for the control group (normal cultured DLD-1 cells) and SPP dose groups; Annexin-V/PI was applied to observe cell apoptosis; XFe24 Extracellular Flux Analyzer was used to detect cell mitochondrial respiratory function and glycolytic function. Results: Comparing with the control, SPP significantly inhibited the proliferation of DLD-1 cells with all the dosage tested (P < 0.01); flow cytometry showed that SPP significantly promoted apoptosis (P < 0.05). Additionally, SPP could significantly inhibited mitochondrial metabolism and glycolysis of DLD-1 cells and decreased cell energy metabolism in all groups treated with different doses. Conclusion: SPP can cause oxidative damage, promote apoptosis, and reduce mitochondrial respiratory and glycolysis rate in colon cancer DLD-1 cells, which reveals that SPP has the potential to serve as the anti-cancer drugs in the future, but further experimental evidence is needed.
文献关键词：
中图分类号：
作者姓名：
Xiaojiao Ji;Jing Wang;Aijin Ma;Duo Feng;Yue He;Wenjie Yan
作者机构：
College of Biochemical Engineering,Beijing Union University,Beijing 100101,China;Institute of Food and Nutrition Development,Ministry of Agriculture and Rural Affairs,Beijing 100125,China;College of Food and Health,Beijing Technology and Business University,Beijing 100048,China
文献出处：
引用格式：
[1]Xiaojiao Ji;Jing Wang;Aijin Ma;Duo Feng;Yue He;Wenjie Yan-.Effects of silkworm pupa protein on apoptosis and energy metabolism in human colon cancer DLD-1 cells)[J].食品科学与人类健康（英文）,2022(05):1171-1176
A类：
pupa,XFe24
B类：
Effects,silkworm,protein,apoptosis,energy,metabolism,human,colon,cancer,DLD,cells,Objective,To,investigate,effects,Bombyx,mori,SPP,proliferation,Methods,CCK,was,used,detect,rate,after,control,normal,cultured,groups,Annexin,applied,observe,Extracellular,Flux,Analyzer,mitochondrial,respiratory,function,glycolytic,Results,Comparing,significantly,inhibited,dosage,tested,flow,cytometry,showed,that,promoted,Additionally,could,glycolysis,decreased,treated,different,doses,Conclusion,cause,oxidative,damage,reduce,which,reveals,has,potential,anti,drugs,future,but,further,experimental,evidence,needed
AB值：
0.465378
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