典型文献
Contractility detection of isolated mouse papillary muscle using myotronic Myostation-Intact device
文献摘要：
Background: To understand the relationship between myocardial contractility and ex-ternal stimuli, detecting ex vivo myocardial contractility is necessary. Methods: We elaborated a method for contractility detection of isolated C57 mouse papillary muscle using Myostation-Intact system under different frequencies, volt-ages, and calcium concentrations. Results: The results indicated that the basal contractility of the papillary muscle was 0.27 ± 0.03 mN at 10 V, 500-ms pulse duration, and 1 Hz. From 0.1 to 1.0 Hz, con-tractility decreased with an increase in frequency (0.45 ± 0.11– 0.10 ± 0.02 mN). The voltage-initiated muscle contractility varied from 3 to 6 V, and the contractility gradu-ally increased as the voltage increased from 6 to 10 V (0.14 ± 0.02– 0.28 ± 0.03 mN). Moreover, the muscle contractility increased when the calcium concentration was increased from 1.5 to 3 mM (0.45 ± 0.17– 1.11 ± 0.05 mN); however, the contractility stopped increasing even when the concentration was increased to 7.5 mM (1.02 ± 0.23 mN). Conclusions: Our method guaranteed the survivability of papillary muscle ex vivo and provided instructions for Myostation-Intact users for isolated muscle contractility investigations.
文献关键词：
中图分类号：
作者姓名：
Hong Lian;Zhuyun Qin;Mengge Wu;Peipei Zuo;Lina Bai;Minjie Lu;Lulu Li;Haitao Zhang
作者机构：
State Key Laboratory of Cardiovascular Disease,Fuwai Hospital,National Center for Cardiovascular Disease,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing,China;Beijing Key Laboratory of Preclinical Research and Evaluation for Cardiovascular Implant Materials,Animal Experimental Center,Fuwai Hospital,National Center for Cardiovascular Disease,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing,China;Adult Surgical Intensive Care Unit,Fuwai Hospital,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing,China;National Health Commission Key Laboratory of Cardiovascular Regenerative Medicine,Fuwai Central-China Hospital,Central-China Subcenter of National Center for Cardiovascular Diseases,Zhengzhou,China
文献出处：
引用格式：
[1]Hong Lian;Zhuyun Qin;Mengge Wu;Peipei Zuo;Lina Bai;Minjie Lu;Lulu Li;Haitao Zhang-.Contractility detection of isolated mouse papillary muscle using myotronic Myostation-Intact device)[J].动物模型与实验医学（英文）,2022(05):445-452
A类：
Contractility,myotronic,Myostation,tractility
B类：
detection,isolated,mouse,papillary,muscle,using,Intact,device,Background,To,understand,relationship,between,myocardial,contractility,ex,ternal,stimuli,detecting,vivo,necessary,Methods,We,elaborated,method,C57,system,different,frequencies,ages,calcium,concentrations,Results,results,indicated,that,basal,was,mN,ms,pulse,duration,From,decreased,frequency,voltage,initiated,varied,from,gradu,ally,increased,Moreover,when,mM,however,stopped,increasing,even,Conclusions,Our,guaranteed,survivability,provided,instructions,users,investigations
AB值：
0.442557
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