典型文献
Hepatocellular carcinoma-derived exosomal miRNA-761 regulates the tumor microenvironment by targeting the SOCS2/JAK2/STAT3 pathway
文献摘要：
BACKGROUND: Exosomes and exosomal microRNAs have been implicated in tumor occurrence and metastasis. Our previous study showed that microRNA-761 (miR-761) is overexpressed in hepatocellular carcinoma (HCC) tissues and that its inhibition affects mitochondrial function and inhibits HCC metastasis. The mechanism by which exosomal miR-761 modulates the tumor microenvironment has not been elucidated.METHODS: Exosomal miR-761 was detected in six cell lines. Cell counting kit-8 (CCK-8) and transwell migration assays were performed to determine the function of exosomal miR-761 in HCC cells. The luciferase reporter assay was used to analyze miR-761 target genes in normal fi broblasts (NFs). The inhibitors AZD1480 and C188-9 were employed to determine the role of the Janus kinase 2/signal transducer and activator of transcription 3 (JAK2/STAT3) signaling pathway in the transformation of cancer-associated fi broblasts (CAFs). RESULTS: In this study, we characterized the mechanism by which miR-761 reprogrammed the tumor microenvironment. We found that HCC-derived exosomal miR-761 was taken up by NFs. Moreover, HCC exosomes aff ected the tumor microenvironment by activating NFs via suppressor of cytokine signaling 2 (SOCS2) and the JAK2/STAT3 signaling pathway. CONCLUSIONS: These results demonstrated that exosomal miR-761 modulated the tumor microenvironment via SOCS2/JAK2/STAT3 pathway-dependent activation of CAFs. Our fi ndings may inspire new strategies for HCC prevention and therapy.
文献关键词：
中图分类号：
作者姓名：
Xiao-hu Zhou;Hao Xu;Chang Xu;Ying-cai Yan;Lin-shi Zhang;Qiang Sun;Wei-lin Wang;Yan-jun Shi
作者机构：
Department of Hepatobiliary and Pancreatic Surgery,the Second Affiliated Hospital of Zhejiang University School of Medicine,Hangzhou 310009,China;Key Laboratory of Precision Diagnosis and Treatment for Hepatobiliary and Pancreatic Tumor of Zhejiang Province,the Second Affiliated Hospital of Zhejiang University School of Medicine,Hangzhou 310009,China
文献出处：
引用格式：
[1]Xiao-hu Zhou;Hao Xu;Chang Xu;Ying-cai Yan;Lin-shi Zhang;Qiang Sun;Wei-lin Wang;Yan-jun Shi-.Hepatocellular carcinoma-derived exosomal miRNA-761 regulates the tumor microenvironment by targeting the SOCS2/JAK2/STAT3 pathway)[J].世界急诊医学杂志（英文）,2022(05):379-385
A类：
broblasts,AZD1480,C188,ndings
B类：
Hepatocellular,carcinoma,derived,exosomal,miRNA,regulates,tumor,microenvironment,by,targeting,SOCS2,JAK2,STAT3,pathway,BACKGROUND,Exosomes,microRNAs,have,been,implicated,occurrence,metastasis,Our,previous,study,showed,that,overexpressed,hepatocellular,HCC,tissues,inhibition,affects,mitochondrial,function,inhibits,mechanism,which,modulates,has,not,elucidated,METHODS,Exosomal,was,detected,six,lines,Cell,counting,kit,CCK,transwell,migration,assays,were,performed,determine,cells,luciferase,reporter,used,analyze,genes,normal,fi,NFs,inhibitors,employed,role,Janus,kinase,transducer,activator,transcription,signaling,transformation,cancer,associated,CAFs,RESULTS,In,this,characterized,reprogrammed,We,found,taken,Moreover,exosomes,activating,via,suppressor,cytokine,CONCLUSIONS,These,results,demonstrated,modulated,dependent,activation,may,inspire,new,strategies,prevention,therapy
AB值：
0.521134
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