BACKGROUND: Patients with sepsis often exhibit an acute inflammatory response, followed by an immunosuppressive phase with a poor immune response. However, the underlying mechanisms have not been fully elucidated. METHODS: We sought to comprehensively characterize the transcriptional changes in neutrophils of patients with sepsis by transcriptome sequencing. Additionally, we conducted a series of experiments, including real-time quantitative polymerase chain reaction (RT-qPCR) and flow cytometry to investigate the role of arginase-1 signaling in sepsis. RESULTS: Through the analysis of gene expression profiles, we identified that the negative regulation of T cell activation signaling was enriched, and the expression of arginase-1 was high in neutrophils from patients with sepsis. Furthermore, we conducted flow cytometry and found that the function of CD8+ T cells in septic patients was impaired. Moreover, neutrophils from septic patients inhibited the percentage of polyfunctional effector CD8+ T cells through arginase-1. Additionally, the proportions of granzyme B+IFN-γ+CD8+ T and TNF-α+IFN-γ+CD8+ T cells increased after inhibition of arginase-1 signaling. CONCLUSION: The impaired effector function of CD8+ T cells could be restored by blocking arginase-1 signaling in patients with sepsis.

Xiao-kang Dai;Zhen-xing Ding;Yuan-yuan Tan;Hua-rui Bao;Dong-yao Wang;Hong Zhang

Department of Emergency,the First Affiliated Hospital of Anhui Medical University,Hefei 230027,China;Department of Hematology,the First Affiliated Hospital of University of Science and Technology of China,Division of Life Sciences and Medicine,Hefei 230001,China;Division of Molecular Medicine,Hefei National Laboratory for Physical Sciences at Microscale,the Chinese Academy of Sciences Key Laboratory of Innate Immunity and Chronic Disease,School of Life Sciences,University of Science and Technology of China,Hefei 230001,China;Anhui Province Key Laboratory of Immunology in Chronic Diseases,Bengbu Medical College,Bengbu 233030,China

世界急诊医学杂志（英文）

[1]Xiao-kang Dai;Zhen-xing Ding;Yuan-yuan Tan;Hua-rui Bao;Dong-yao Wang;Hong Zhang-.Neutrophils inhibit CD8+ T cells immune response by arginase-1 signaling in patients with sepsis)[J].世界急诊医学杂志（英文）,2022(04):266-273

B+IFN,+CD8+

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