典型文献
Immobilization of Chitosanase on Magnetic Nanoparticles:Preparation,Characterization and Properties
文献摘要：
Chitosanase could cleave β-1,4-linkage of chitosan to produce chitooligosaccharides(COS)with diverse biological ac-tivities.However,there are many limitations on the use of free chitosanase in industrial production.Enzyme immobilization is gener-ally considered a valuable strategy in industrial-scale applications.In this study,the chitosanase Csn-BAC from Bacillus sp.MD-5 was immobilized on Fe3O4-SiO2 magnetic nanoparticles(MNPs)to enhance its properties,which could be recovered easily from reaction media using magnetic separation.The activities of Csn-BAC immobilized with MNPs(MNPs@Csn-BAC)were de-termined with temperature and pH,and the thermal-and pH-stabilities,respectively.The reusability of the MNPs@Csn-BAC was determined in repeated reaction cycles.Immobilization enhanced the thermal and pH stability of Csn-BAC compared with the free enzyme.After eight reaction cycles using MNPs@Csn-BAC,the residual enzyme activity was 72.15％.Finally,the amount of COS released by MNPs@Csn-BAC was 1.86 times higher than that of the free Csn-BAC in the catalytic performance experiment.The immobilized Csn-BAC exhibits broad application prospects in the production of COS.
文献关键词：
中图分类号：
作者姓名：
CHENG Yimeng;LI Zhaoyue;SUN Huihui;ZHAO Ling;LIU Zhen;CAO Rong;LIU Qi;MAO Xiangzhao
作者机构：
College of Food Science and Engineering,Ocean University of China,Qingdao 266003,China;Department of Food Engineering and Nutrition,Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Qingdao 266071,China;Laboratory for Marine Drugs and Bioproducts of Qingdao,National Laboratory for Marine Science and Technology,Qingdao 266237,China
文献出处：
引用格式：
[1]CHENG Yimeng;LI Zhaoyue;SUN Huihui;ZHAO Ling;LIU Zhen;CAO Rong;LIU Qi;MAO Xiangzhao-.Immobilization of Chitosanase on Magnetic Nanoparticles:Preparation,Characterization and Properties)[J].中国海洋大学学报（自然科学英文版）,2022(05):1381-1388
A类：
Chitosanase,chitosanase
B类：
Immobilization,Magnetic,Nanoparticles,Preparation,Characterization,Properties,could,cleave,linkage,produce,chitooligosaccharides,COS,diverse,biological,However,there,many,limitations,use,free,industrial,production,Enzyme,immobilization,gener,considered,valuable,strategy,scale,applications,In,this,study,Csn,BAC,from,Bacillus,MD,was,immobilized,Fe3O4,SiO2,magnetic,nanoparticles,MNPs,properties,which,be,recovered,easily,reaction,media,using,separation,activities,were,temperature,thermal,stabilities,respectively,reusability,determined,repeated,cycles,enhanced,stability,compared,enzyme,After,eight,residual,activity,Finally,amount,released,by,times,higher,than,that,catalytic,performance,experiment,exhibits,broad,prospects
AB值：
0.475258
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