Nervous necrosis virus(NNV)can infect more than 120 fish species worldwide and has caused high mortality and sig-nificant economic losses to the aquaculture industry.Among different genotypes of NNV,the red-grouper nervous necrosis virus(RGNNV)is the most widely distributed one with the highest number of susceptible fish species.In this study,the capsid protein(Cp)gene of RGNNV was recombined and expressed in Escherichia coli strain BL21(DE3)and the recombinant Cp(rCp)was used as an immunogen to produce monoclonal antibodies(MAbs)through hybridoma cell fusion technology.Three MAbs were produced and characterized by indirect enzyme-linked immunosorbent assay(ELISA),western blotting,and immunofluorescence assay(IFA).Wes-tern blotting result showed that the MAbs could specifically react with the capsid protein of RGNNV.The result of IFA showed that the MAbs could recognize virions in RGNNV-infected grouper spleen(GS)cells.These results indicate that the MAbs can specifi-cally recognize RGNNV virions and can be used to produce a rapid detection method.This study provides a foundation for further studies on the rapid diagnosis of RGNNV and its infection mechanisms.

QIN Yinghui;LIU Jiaoyun;LU Yuanan;LIU Xueqin

College of Fisheries,Huazhong Agricultural University,Wuhan 430070,China;Hubei Engineering Technology Research Center for Aquatic Animal Diseases Control and Prevention,Wuhan 430070,China;Engineering Research Center of Green Development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt,Ministry of Education,Wuhan 430070,China;Department of Public Health Sciences,University of Hawaii at Manoa,Honolulu,HI 96822,USA

中国海洋大学学报（自然科学英文版）

[1]QIN Yinghui;LIU Jiaoyun;LU Yuanan;LIU Xueqin-.Generation and Characterization of Monoclonal Antibodies Against Red-Spotted Grouper Nervous Necrosis Virus(RGNNV))[J].中国海洋大学学报（自然科学英文版）,2022(04):1061-1067

Grouper,NNV,rCp,hybridoma

Generation,Characterization,Monoclonal,Antibodies,Against,Red,Spotted,Nervous,Necrosis,Virus,RGNNV,necrosis,virus,more,than,fish,species,worldwide,has,caused,mortality,sig,nificant,economic,losses,aquaculture,industry,Among,different,genotypes,red,grouper,nervous,most,widely,distributed,one,highest,number,susceptible,In,this,study,capsid,protein,gene,was,recombined,expressed,Escherichia,coli,strain,BL21,DE3,recombinant,immunogen,monoclonal,antibodies,MAbs,through,fusion,technology,Three,were,produced,characterized,by,indirect,enzyme,linked,immunosorbent,assay,western,blotting,immunofluorescence,IFA,Wes,showed,that,could,specifically,react,recognize,virions,infected,spleen,GS,cells,These,results,indicate,rapid,detection,method,This,provides,foundation,further,studies,diagnosis,its,infection,mechanisms

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