Continued innovation in screening methodologies remains important for the discovery of high-quality multiactive fungi,which have been of great significance to the development of new drugs.Mangrove-derived fungi,which are well recognized as pro-lific sources of natural products,are worth sustained attention and further study.In this study,118 fungi,which mainly included As-pergillus spp.(34.62％)and Penicillium spp.(15.38％),were isolated from the mangrove ecosystem of the Maowei Sea,and 83.1％of the cultured fungi showed at least one bioactivity in four antibacterial and three antioxidant assays.To accurately evaluate the fungal bioactivities,the fungi with multiple bioactivities were successfully evaluated and screened by principal component analysis(PCA),and this analysis provided a dataset for comparing and selecting multibioactive fungi.Among the 118 mangrove-derived fungi tested in this study,Aspergillus spp.showed the best comprehensive activity.Fungi such as A.clavatonanicus,A.flavipes and A.citrinoterreus,which exhibited high comprehensive bioactivity as determined by the PC A,have great potential in the exploitation of natural products and the development of new drugs.This study demonstrated the first use of PCA as a time-saving,scientific method with a strong ability to evaluate and screen multiactive fungi,which indicated that this method can affect the discovery and development of new drugs.

YANG Zonglin;SHI Yaqi;LI Pinglin;PAN Kanghong;LI Guoqiang;LI Xianguo;YAO Shuo;ZHANG Dahai

Key Laboratory of Marine Chemistry Theory and Technology(Ocean University of China),Ministry of Education,Qingdao 266100,China;College of Chemistry and Chemical Engineering,Ocean University of China,Qingdao 266100,China;Key Laboratory of Marine Drugs,Chinese Ministry of Education,School of Medicine and Pharmacy,Ocean University of China,Qingdao 266100,China;Laboratory of Marine Drugs and Biological Products,National Laboratory for Marine Science and Technology,Qingdao 266100,China

中国海洋大学学报（自然科学英文版）

[1]YANG Zonglin;SHI Yaqi;LI Pinglin;PAN Kanghong;LI Guoqiang;LI Xianguo;YAO Shuo;ZHANG Dahai-.Application of Principal Component Analysis(PC A)to the Evaluation and Screening of Multiactivity Fungi)[J].中国海洋大学学报（自然科学英文版）,2022(03):763-772

Multiactivity,multiactive,Mangrove,lific,Maowei,multibioactive,clavatonanicus,citrinoterreus

Application,Principal,Component,Analysis,Evaluation,Screening,Fungi,Continued,innovation,screening,methodologies,remains,important,discovery,high,quality,fungi,which,have,been,great,significance,development,new,drugs,derived,are,well,recognized,sources,natural,products,worth,sustained,attention,further,study,In,this,mainly,included,spp,Penicillium,were,isolated,from,mangrove,ecosystem,Sea,cultured,showed,least,bioactivity,four,antibacterial,three,antioxidant,assays,To,accurately,fungal,bioactivities,multiple,successfully,evaluated,screened,by,principal,component,analysis,provided,dataset,comparing,selecting,Among,tested,Aspergillus,best,comprehensive,such,flavipes,exhibited,determined,potential,exploitation,This,demonstrated,first,use,saving,scientific,strong,ability,indicated,that,affect

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