The mRNA modification N6-methyladenosine (m6A) plays vital roles in plant development and biotic and abiotic stress responses. The RNA m6A demethylase ALKBH9B can remove m6A in alfalfa mosaic virus RNA and plays roles in alfalfa mosaic virus infection in Arabidopsis. However, it is unknown whether ALKBH9B also exhibits demethylation activity and has a biological role in endogenous plant mRNA. We demonstrated here that mRNA m6A modification is induced by the phytohormone abscisic acid (ABA) and that ALKBH9B has m6A demethylation activity on endogenous mRNA. Knocking out ALKBH9B led to hypersensitivity to ABA treatment during seed germination and early seedling development. We further showed that ALKBH9B removes the m6A modification in the ABA INSENSITIVE 1 (ABI1) and BRI1-EMS-SUPPRESSOR 1 (BES1) transcripts fol-lowing ABA treatment, affecting the stability of these mRNAs. Furthermore, we determined that ALKBH9B acts genetically upstream of the transcription factors ABI3 and ABI5, and its regulatory function in ABA responses depended on ABI3 and ABI5. Our findings reveal the important roles of the m6A modification in ABA responses and highlight the role of ALKBH9B-mediated m6A demethylation in regulating ABA re-sponses post-transcriptionally.

Synthetic and Functional Biomolecules Center,Beijing National Laboratory for Molecular Sciences,Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education,Department of Chemical Biology,College of Chemistry and Molecular Engineering,Peking University,Beijing 100871,China;Peking-Tsinghua Center for Life Sciences,Beijing 100871,China

[1]Jun Tang;Junbo Yang;Qiang Lu;Qian Tang;Shuyan Chen;Guifang Jia-.The RNA N6-methyladenosine demethylase ALKBH9B modulates ABA responses in ArabidopsisOO)[J].植物学报（英文版）,2022(12):2361-2373

ALKBH9B,ArabidopsisOO,ABI1

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