Necrostatin-1,an inhibitor of necroptosis,can effectively inhibit necrotic apoptosis in neurological diseases,which results in the inhibition of inflammation,endoplasmic reticulum stress,and reactive oxygen species production and substantial improvement of neurological function.However,the effects of necrostatin-1 on intraventricular hemorrhage(IVH)remain unknown.In this study,we established a mouse model of IVH by injecting autologous blood into the lateral ventricle of the brain.We also injected necrostatin-1 into the lateral ventricle one hour prior to IVH induction.We found that necrostatin-1 effectively reduced the expression levels of the necroptosis markers receptor-interacting protein kinase(RIP)1,RIP3,mixed lineage kinase domain-like protein(MLKL),phosphorylated(p)-RIP3,and p-MLKL and the levels of interleukin-1β,interleukin-6,and tumor necrosis factor-α in the surrounding areas of the lateral ventricle.However,necrostatin-1 did not reduce ependymal ciliary injury or brain water content.These findings suggest that necrostatin-1 can prevent local inflammation and microglial activation induced by IVH but does not greatly improve prognosis.

Chang Liu;Yi Cao;Hao-Xiang Wang;Long Zhao;Ya-Xing Chen;Kun-Hong Zhong;Gao-Wei Li;Guo-Qing Wang;Ke-Ru Huang;Ai-Ping Tong;Liang-Xue Zhou

Department of Neurosurgery,West China Medical School,West China Hospital,Sichuan University,Chengdu,Sichuan Province,China;Department of Neurosurgery,Chengdu Second People's Hospital,Chengdu,Sichuan Province,China;Department of Neurosurgery,Affiliated Hospital of North Sichuan Medical College,Nanchong,Sichuan Province,China;State Key Laboratory of Biotherapy,West China Medical School,Sichuan University,Chengdu,Sichuan Province,China

中国神经再生研究（英文版）

[1]Chang Liu;Yi Cao;Hao-Xiang Wang;Long Zhao;Ya-Xing Chen;Kun-Hong Zhong;Gao-Wei Li;Guo-Qing Wang;Ke-Ru Huang;Ai-Ping Tong;Liang-Xue Zhou-.Necrostatin-1 decreases necroptosis and inflammatory markers after intraventricular hemorrhage in mice)[J].中国神经再生研究（英文版）,2022(12):2710-2716

ependymal

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