Retinitis pigmentosa is a retinal disease characterized by photoreceptor degeneration. There is currently no effective treatment for retinitis pigmentosa. Although a mixture of lutein and other antioxidant agents has shown promising effects in protecting the retina from degeneration, the role of lutein alone remains unclear. In this study, we administered intragastric lutein to Pde6brd10 model mice, which display degeneration of retinal photoreceptors, on postnatal days 17 (P17) to P25, when rod apoptosis reaches peak. Lutein at the optimal protective dose of 200 mg/kg promoted the survival of photoreceptors compared with vehicle control. Lutein increased rhodopsin expression in rod cells and opsin expression in cone cells, in line with an increased survival rate of photoreceptors. Functionally, lutein improved visual behavior, visual acuity, and retinal electroretinogram responses in Pde6brd10 mice. Mechanistically, lutein reduced the expression of glial fibrillary acidic protein in Müller glial cells. The results of this study confirm the ability of lutein to postpone photoreceptor degeneration by reducing reactive gliosis of Müller cells in the retina and exerting anti-inflammatory effects. This study was approved by the Laboratory Animal Ethics Committee of Jinan University (approval No. LACUC-20181217-02) on December 17, 2018.

Hui-Jun Zhang;Xiao-Bin Liu;Xiong-Min Chen;Qi-Hang Kong;Yu-Sang Liu;Kwok-Fai So;Jian-Su Chen;Ying Xu;Xue-Song Mi;Shi-Bo Tang

Department of Ophthalmology,the First Affiliated Hospital of Jinan University,Guangzhou,Guangdong Province,China;Department of Ophthalmology,Guangzhou Panyu Central Hospital,Guangzhou,Guangdong Province,China;Guangdong-Hong Kong-Macau Institute of CNS Regeneration,Jinan University,Guangzhou,Guangdong Province,China;Aier Academician Station,Changsha,Hunan Province,China;Key Laboratory of CNS Regeneration(Jinan University),Ministry of Education,Guangzhou,Guangdong Province,China;Co-Innovation Center of Neuroregeneration,Nantong University,Nantong,Jiangsu Province,China;Laboratory of Retinal Cell Biology,Aier Eye Institute,Changsha,Hunan Province,China

中国神经再生研究（英文版）

[1]Hui-Jun Zhang;Xiao-Bin Liu;Xiong-Min Chen;Qi-Hang Kong;Yu-Sang Liu;Kwok-Fai So;Jian-Su Chen;Ying Xu;Xue-Song Mi;Shi-Bo Tang-.Lutein delays photoreceptor degeneration in a mouse model of retinitis pigmentosa)[J].中国神经再生研究（英文版）,2022(07):1596-1603

Retinitis,Pde6brd10,opsin,gliosis,LACUC

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