Neuroinflammation plays a significant role in inducing depression-like behavior.Tetrahedral DNA nanos-tructures(TDNs)are molecules that exhibit anti-inflammatory properties and can effectively penetrate the blood-brain barrier.Thus,researchers have hypothesized that TDNs regulate the secretion of proin-flammatory cytokines and consequently alleviate depression-like behavior.To test this hypothesis,we investigated the effect of TDNs on the depression-like behavior of C57 mice induced by lipopolysaccha-ride(LPS).We performed open-field,tail suspension,and sucrose preference tests on LPS-and LPS/TDN-treated mice.The results indicated that the injection of TDNs into LPS-treated mice resulted in increased velocity,center zone duration,frequency to the center zone,and sucrose preference,and decreased im-mobility time.Immunofluorescence results indicated that peripheral administration of LPS in the mice activated inflammation,which culminated in distinct depression-like behavior.However,TDNs effectively alleviated the inflammation and depression-like behavior through the reduction of the expression levels of proinflammatory cytokines,such as interleukin-1β and tumor necrosis factor-α in the brain.Addition-ally,TDNs normalized the expression level of microglia cell activation markers,such as ionized calcium binding adaptor molecule 1,in the hippocampus of mice.These results indicated that TDNs attenuated the LPS-induced secretion of inflammatory factors and consequently alleviated depression-like behavior.

Xiao Yang;Fan Zhang;Yue Du;Weitong Cui;Yikai Dou;Yunfeng Lin;Zhihe Zhao;Xiaohong Ma

Psychiatric Laboratory and Mental Health Center,West China Hospital of Sichuan University,Chengdu 610041,China;State Key Laboratory of Oral Diseases,National Clinical Research Center for Oral Diseases,West China Hospital of Stomatology,Sichuan University,Chengdu 610041,China;Physical Examination Center,West China Hospital,Sichuan University,Chengdu 610041,China;College of Biomedical Engineering,Sichuan University,Chengdu 610041,China

中国化学快报（英文版）

[1]Xiao Yang;Fan Zhang;Yue Du;Weitong Cui;Yikai Dou;Yunfeng Lin;Zhihe Zhao;Xiaohong Ma-.Effect of tetrahedral DNA nanostructures on LPS-induced neuroinflammation in mice)[J].中国化学快报（英文版）,2022(04):1901-1906

TDNs,lipopolysaccha,culminated

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