典型文献
Rice transcription factors OsLBD37/38/39 regulate nitrate uptake by repressing OsNRT2.1/2.2/2.3 under high-nitrogen conditions
文献摘要：
Nitrate (NO3-) uptake involves a finely regulated and complex multilevel response system. Elucidating the molecular mechanism of nitrate uptake may lead to improving the growth and productivity of plants in the presence of dynamic variation in nitrate concentration. In this study, we identified three lateral organ boundaries domain (LBD) transcription factors, OsLBD37, OsLBD38, and OsLBD39, as regulators of nitrate uptake in response to nitrogen (N) availability. OsLBD37, OsLBD38, and OsLBD39 were induced by ammo-nium and glutamine in rice roots. Individual or collective knockout of OsLBD37, OsLBD38, and OsLBD39 led to increased concentrations of nitrate and increased expression of OsNRT2.1, OsNRT2.2, and OsNRT2.3 respectively under high-N conditions, whereas overexpression of each of these three LBD genes produced opposite effects where N accumulation was reduced. Dual-luciferase reporter assay further confirmed that OsLBD37, OsLBD38, and OsLBD39 possessed transcription inhibitory activities in rice protoplast cells, downregulating the expression of OsNRT2.1/OsNRT2.2/OsNRT2.3. Yeast two-hybrid and bimolecular fluo-rescence complementation assays showed that OsLBD37 interacted with OsLBD37, OsLBD38, and OsLBD39 in the nucleus. Together, these results show that OsLBD37, OsLBD38, and OsLBD39 collaborate to inhibit the expression of OsNRT2.1/OsNRT2.2/OsNRT2.3 transporters under N-sufficient conditions, thereby help-ing rice plants avoid excessive nitrate accumulation that may affect their growth.
文献关键词：
中图分类号：
作者姓名：
Xinxin Zhu;Dujun Wang;Lijuan Xie;Tao Zhou;Jingyi Zhao;Qian Zhang;Meng Yang;Wenjuan Wu;Xingming Lian
作者机构：
National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research(Wuhan),Huazhong Agricultural University,Wuhan 430070,Hubei,China
文献出处：
引用格式：
[1]Xinxin Zhu;Dujun Wang;Lijuan Xie;Tao Zhou;Jingyi Zhao;Qian Zhang;Meng Yang;Wenjuan Wu;Xingming Lian-.Rice transcription factors OsLBD37/38/39 regulate nitrate uptake by repressing OsNRT2.1/2.2/2.3 under high-nitrogen conditions)[J].作物学报（英文版）,2022(06):1623-1632
A类：
OsLBD37,OsNRT2,OsLBD38,OsLBD39,protoplast
B类：
Rice,transcription,factors,nitrate,uptake,repressing,under,high,nitrogen,conditions,Nitrate,NO3,involves,finely,regulated,complex,multilevel,response,system,Elucidating,mechanism,may,lead,improving,growth,productivity,plants,presence,dynamic,variation,this,study,identified,three,lateral,organ,boundaries,domain,regulators,availability,were,induced,ammo,nium,glutamine,rice,roots,Individual,collective,knockout,led,increased,concentrations,respectively,whereas,overexpression,each,these,genes,produced,opposite,effects,accumulation,was,reduced,Dual,luciferase,reporter,further,confirmed,that,possessed,inhibitory,activities,cells,downregulating,Yeast,two,hybrid,bimolecular,fluo,rescence,complementation,assays,showed,interacted,nucleus,Together,results,collaborate,transporters,sufficient,thereby,help,avoid,excessive,affect,their
AB值：
0.44898
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