Robinia pseudoacacia is an important afforesta-tion tree introduced to China in 1878. In the present study, we examined the genetic diversity among 687 strains rep-resenting four improved varieties and two secondary prov-enances, comprising 641 clones and 46 seedlings. Ninety-one simple sequence repeats (SSRs) were selected through segregation analysis and polymorphism characterization, and all sampled individuals were genotyped using well-characterized SSR markers. After excluding loci with non-neutral equilibrium, missing locus data and null alleles, we used 36 primer pairs to assess the genetic diversity of these germplasm resources, revealing vast genetic differentiation among the samples, with an average of 8.352 alleles per locus and a mean Shannon′s index of 1.302. At the popula-tion level, the partitioning of variability was assessed using analysis of molecular variance, which revealed 93％ and 7％ variation within and among collection sites, respectively. Four clusters were detected using structure analysis, indi-cating a degree of genetic differentiation among the six populations. Insights into the genetic diversity and structure of R. pseudoacacia provide a theoretical basis for the con-servation, breeding and sustainable development in China.

Qi Guo;Sen Cao;Li Dong;Xiuyu Li;Jiangtao Zhang;Yuanshuai Zhang;Zijie Zhang;Yuhan Sun;Cui Long;Yingming Fan;Chao Han;Peiyao Han;Xiaodi Liu;Yun Li

Beijing Advanced Innovation Center for Tree Breeding By Molecular Design,Engineering Technology Research Center of Black Locust of National Forestry and Grassland Administration,College of Biological Sciences and Technology,National Engineering Laboratory for Tree Breeding,Beijing Forestry University,Beijing 100083, P.R. China;College of Agriculture,Henan University of Science and Technology,Luoyang 471023,P.R. China;Henan Academy of Forestry,Zhengzhou 450008,P.R. China;Feixian County State Owned Daqingshan Forest Farm, Linyi 273400,P.R. China

林业研究（英文版）

[1]Qi Guo;Sen Cao;Li Dong;Xiuyu Li;Jiangtao Zhang;Yuanshuai Zhang;Zijie Zhang;Yuhan Sun;Cui Long;Yingming Fan;Chao Han;Peiyao Han;Xiaodi Liu;Yun Li-.Genetic diversity and population structure of Robinia pseudoacacia from six improved variety bases in China as revealed by simple sequence repeat markers)[J].林业研究（英文版）,2022(02):611-621

afforesta,prov,enances

Genetic,diversity,structure,Robinia,pseudoacacia,from,six,improved,variety,bases,China,revealed,by,simple,sequence,markers,important,tree,introduced,present,study,examined,genetic,among,strains,resenting,four,varieties,two,secondary,comprising,clones,seedlings,Ninety,repeats,SSRs,were,selected,through,segregation,analysis,polymorphism,characterization,sampled,individuals,genotyped,using,well,characterized,After,excluding,loci,neutral,equilibrium,missing,locus,data,null,alleles,used,primer,pairs,these,germplasm,resources,revealing,vast,differentiation,samples,average,per,mean,Shannon,At,level,partitioning,variability,was,assessed,molecular,variance,which,variation,within,collection,sites,respectively,Four,clusters,detected,cating,degree,populations,Insights,into,provide,theoretical,basis,servation,breeding,sustainable,development

0.603451