In view of the accumulation of nanoplastics(NPs)in the food chain of environment and animals,and the good adsorption properties of nano-plastics to toxic substances,it is necessary to explore the influence of NPs in living organisms.In this study,single and joint toxicological effects of polystyrene nanoplastics(PS-NPs,size 80 nm)and polychlorinated biphenyls(PCBs),were explored in freshwater aquatic animal model zebrafish(Danio rerio).Our study found that exposure to single PS-NPs induced mild acute toxicity,albeit the combined exposure of PS-NPs and polychlorinated biphenyls aggravated the toxicity of PCBs in a dose-dependent manner.Results from gene expression profiling showed that NPs exposure could acti-vate detoxification process,resulting in a slight up-regulation of antioxidant genes(sod1,gstp1),bone development genes(bmp2,bmp4)and cardiac gene(tbx20);while PCBs suppressed the detoxification through down-regulation of these genes,and the addition of NPs will exacerbate the impact of PCBs on gene suppression.Importantly,the results of in vivo purification experiments found that NPs showed prolonged retention in liver,intestine and gills of zebrafish and they might have crossed biological barrier and accumulate in lipid-rich tissues and excretion does not appear as the significant pathway for their elimination.In conclusion,the toxic effects of polychlorinated biphenyls on chorionic protected embryos were not significant as zebrafish chorion plays an important role in resisting the invasion of pollutants;PCBs can seriously damage the bone and heart development of zebrafish,while the presence of NPs sig-nificantly enhanced the toxicity of PCBs in zebrafish,which is an alarming concern for growing NPs levels and ecological safety in aquatic environment.

Yizheng Li;Shulin Liu;Qiuping Wang;Yanling Zhang;Xikun Chen;Lei Yan;Muhammad Junaid;Jun Wang

College of Marine Sciences,South China Agricultural University,Guangzhou 510642,China;Southern Marine Science and Engineering Guangdong Laboratory(Zhuhai),Zhuhai 528478,China;Institute of Eco-Environmental Research,Guangxi Academy of Sciences,Nanning 530007,China

地学前缘（英文版）

[1]Yizheng Li;Shulin Liu;Qiuping Wang;Yanling Zhang;Xikun Chen;Lei Yan;Muhammad Junaid;Jun Wang-.Polystyrene nanoplastics aggravated ecotoxicological effects of polychlorinated biphenyls in on zebrafish(Danio rerio)embryos)[J].地学前缘（英文版）,2022(03):168-176

sod1,gstp1,bmp2,bmp4,tbx20

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