典型文献
Screening and characterization of proteases produced by deep-sea cold seep bacteria
文献摘要：
Fifty protease-producing strains were screened from sediment of deep-sea cold seep,and divided into four different categories:Bacillus,Pseudoalteromonas,Vibrio,and Alteromonas according to the sequences of 16s rRNA.Their abilities to produce protease,amylase,and lipase were determined,and a Bacillus strain gcc-1 displayed very strong alkaline protease activity and stability under different thermal and acidic conditions.The purification of the protease produced by strain gcc-1 was carried out by precipitation with ammonium sulfate,and sequentially chromatographed by anion exchange column and gel filtration.The purified protease showed a single band at the molecular weight of 28 kDa by SDS-PAGE.The characterization results show that the purified protease exhibited a considerable activity and stability in a wide thermal range of 10-80℃and a wide acidic range of pH 6.5-11.5,and displayed highest activity at 40℃and pH 8.5.Notably,the protease still maintained high activity even at low to 10℃.Furthermore,the protease exhibited good stability in presence of different surfactants,organic solvents,oxidizing agent H2O2,and commercial detergents.Therefore,the protease produced by gcc-1 is a cold active and high stable enzyme,and has a promising potential in laundry detergent as an additive.
文献关键词：
中图分类号：
作者姓名：
Chenchen GUO;Chaomin SUN;Shimei WU
作者机构：
College of Life Sciences,Qingdao University,Qingdao 266071,China;CAS Key Laboratory of Experimental Marine Biology,Institute of Oceanology,Chinese Academy of Sciences,Qingdao 266071,China
文献出处：
引用格式：
[1]Chenchen GUO;Chaomin SUN;Shimei WU-.Screening and characterization of proteases produced by deep-sea cold seep bacteria)[J].海洋湖沼学报（英文版）,2022(02):678-689
A类：
gcc,chromatographed
B类：
Screening,characterization,proteases,produced,by,deep,sea,cold,seep,bacteria,Fifty,producing,strains,were,screened,from,sediment,divided,into,four,different,categories,Bacillus,Pseudoalteromonas,Vibrio,Alteromonas,according,sequences,16s,rRNA,Their,abilities,amylase,lipase,determined,displayed,very,strong,alkaline,activity,stability,under,thermal,acidic,conditions,purification,was,carried,out,precipitation,ammonium,sulfate,sequentially,anion,exchange,column,gel,filtration,purified,showed,single,band,molecular,weight,kDa,SDS,PAGE,results,that,exhibited,considerable,wide,range,highest,Notably,still,maintained,even,low,Furthermore,good,presence,surfactants,organic,solvents,oxidizing,agent,H2O2,commercial,detergents,Therefore,active,stable,enzyme,has,promising,potential,laundry,additive
AB值：
0.577886
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