Lipid metabolism is very important for meat quality in pigs.Accumulating evidence shows that gut microbiota can contribute to this physiological process.However,the gut microbiota that function in lipid metabolism and adipogenesis remains unclear.Here,we compared the characteristics of fat deposition and gut microbial community between Laiwu pigs and Duroc×(Landrace×Yorkshire)(DLY)pigs.Fecal microbiota transplantation(FMT)was performed to determine the possible impact of gut microbiota on lipid metabolism in pigs.An integrated analysis of the gut microbiome and lipidome of the small in-testine,plasma,and liver was conducted to investigate the effects of FMT on host lipid metabolism.The comparative analysis of the gut microbiome showed higher abundance of Bacteroidetes(P=0.0018)while lower abundance of Firmicutes(P=0.012)in Laiwu pigs,and the microbial composition can be transferred from Laiwu pigs into DLY pigs.Transmission electron microscope and Oil red-O staining were performed to analyze the effects of FMT on lipid deposition in liver,the main target organ for lipid metabolism.The results showed that FMT significantly increased the number of lipid droplets(P=0.0035)and lipid accumulation(P=0.0026)in liver.Furthermore,integrated multi-tissues lipidome analysis demonstrated that the fatty acyls and glycerophospholipids were significantly increased(P＜0.01)in intestine and liver,while glycerolipids and fatty acyls were reduced(P＜0.01)in plasma.In the small intestine,FMT increased(P＜0.01)the relative abundance of polyketides and prenol lipids but reduced(P＜0.01)the saccharolipids.Correlation analysis revealed the potential interactions between microbiota and lipid metabolites.Together,our results indicated that the gut microbiota may regulate the lipid metabolism and enhance the accumulation of lipid droplets in the liver of pigs.

Chunlin Xie;Xiaoyan Zhu;Baoyang Xu;Yaorong Niu;Xuelei Zhang;Libao Ma;Xianghua Yan

State Key Laboratory of Agricultural Microbiology,Hubei Hongshan Laboratory,Frontiers Science Center for Animal Breeding and Sustainable Production,College of Animal Sciences and Technology,Huazhong Agricultural University,Wuhan,Hubei 430070,China;The Cooperative Innovation Center of Sustainable Pig Production,Wuhan,Hubei 430070,China;Hubei Provincial Engineering Laboratory for Pig Precision Feeding and Feed Safety Technology,Wuhan,Hubei 430070,China

动物营养（英文）

[1]Chunlin Xie;Xiaoyan Zhu;Baoyang Xu;Yaorong Niu;Xuelei Zhang;Libao Ma;Xianghua Yan-.Integrated analysis of multi-tissues lipidome and gut microbiome reveals microbiota-induced shifts on lipid metabolism in pigs)[J].动物营养（英文）,2022(03):280-293

Laiwu,testine,acyls,prenol,saccharolipids

Integrated,analysis,multi,tissues,lipidome,gut,microbiome,reveals,microbiota,induced,shifts,metabolism,pigs,Lipid,very,important,meat,quality,Accumulating,evidence,shows,that,contribute,this,physiological,process,However,function,adipogenesis,remains,unclear,Here,compared,characteristics,deposition,microbial,community,between,Duroc,Landrace,Yorkshire,DLY,Fecal,transplantation,FMT,was,performed,determine,possible,impact,An,integrated,small,plasma,liver,conducted,investigate,effects,host,comparative,showed,higher,abundance,Bacteroidetes,while,lower,Firmicutes,composition,transferred,from,into,Transmission,electron,microscope,Oil,staining,were,analyze,target,organ,results,significantly,increased,number,droplets,accumulation,Furthermore,demonstrated,fatty,glycerophospholipids,intestine,glycerolipids,reduced,relative,polyketides,Correlation,revealed,potential,interactions,metabolites,Together,our,indicated,may,regulate,enhance

0.424782